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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has been noticed in many tumors, which results in chromosome instability, and thus could lead to tumorigenesis. This protein was uncovered to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a essential purpose in promoting cell-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have been described. Two further splice variants had been noted but in depth nucleotide sequence details isn't however available.

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Blocking peptides are peptides that bind particularly into the focus on antibody and block antibody binding. These peptide usually has the epitope recognized because of the antibody.

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This guide outlines additional achievable optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...

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This information outlines additional attainable optimization of mobile and immunoassay parameters to be certain the very best outcomes are...

Antibodies sure to the blocking peptide no longer bind to your epitope about the goal protein. By comparing the staining with the blocked antibody vs . the antibody on your own, you can see which staining is specific.

Goal Data Cyclin E belongs into the extremely conserved cyclin spouse and children, whose members are characterized by a remarkable periodicity in protein abundance with the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins show distinct expression and degradation patterns which add to your temporal coordination of each and every mitotic function. Cyclin E forms a posh with and features to be a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S transition. This protein accumulates in the G1-S section boundary and is particularly degraded as cells development by S stage.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...

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